1,755 research outputs found
Timing verification of dynamically reconfigurable logic for Xilinx Virtex FPGA series
This paper reports on a method for extending existing VHDL design and verification software available for the Xilinx Virtex series of FPGAs. It allows the designer to apply standard hardware design and verification tools to the design of dynamically reconfigurable logic (DRL). The technique involves the conversion of a dynamic design into multiple static designs, suitable for input to standard synthesis and APR tools. For timing and functional verification after APR, the sections of the design can then be recombined into a single dynamic system. The technique has been automated by extending an existing DRL design tool named DCSTech, which is part of the Dynamic Circuit Switching (DCS) CAD framework. The principles behind the tools are generic and should be readily extensible to other architectures and CAD toolsets. Implementation of the dynamic system involves the production of partial configuration bitstreams to load sections of circuitry. The process of creating such bitstreams, the final stage of our design flow, is summarized
Geometry dominated fluid adsorption on sculptured substrates
Experimental methods allow the shape and chemical composition of solid
surfaces to be controlled at a mesoscopic level. Exposing such structured
substrates to a gas close to coexistence with its liquid can produce quite
distinct adsorption characteristics compared to that occuring for planar
systems, which may well play an important role in developing technologies such
as super-repellent surfaces or micro-fluidics. Recent studies have concentrated
on adsorption of liquids at rough and heterogeneous substrates and the
characterisation of nanoscopic liquid films. However, the fundamental effect of
geometry has hardly been addressed. Here we show that varying the shape of the
substrate can exert a profound influence on the adsorption isotherms allowing
us to smoothly connect wetting and capillary condensation through a number of
novel and distinct examples of fluid interfacial phenomena. This opens the
possibility of tailoring the adsorption properties of solid substrates by
sculpturing their surface shape.Comment: 6 pages, 4 figure
Training emergency services’ dispatchers to recognise stroke: an interrupted time-series analysis
Background: Stroke is a time-dependent medical emergency in which early presentation to specialist care reduces death and dependency. Up to 70% of all stroke patients obtain first medical contact from the Emergency Medical Services (EMS). Identifying ‘true stroke’ from an EMS call is challenging, with over 50% of strokes being misclassified.
The aim of this study was to evaluate the impact of the training package on the recognition of stroke by Emergency Medical Dispatchers (EMDs).
Methods: This study took place in an ambulance service and a hospital in England using an interrupted time-series
design. Suspected stroke patients were identified in one week blocks, every three weeks over an 18 month period,
during which time the training was implemented. Patients were included if they had a diagnosis of stroke (EMS or
hospital). The effect of the intervention on the accuracy of dispatch diagnosis was investigated using binomial
(grouped) logistic regression.
Results: In the Pre-implementation period EMDs correctly identified 63% of stroke patients; this increased to 80%
Post-implementation. This change was significant (p=0.003), reflecting an improvement in identifying stroke patients
relative to the Pre-implementation period both the During-implementation (OR=4.10 [95% CI 1.58 to 10.66]) and Post-implementation (OR=2.30 [95% CI 1.07 to 4.92]) periods. For patients with a final diagnosis of stroke who had been dispatched as stroke there was a marginally non-significant 2.8 minutes (95% CI −0.2 to 5.9 minutes, p=0.068)reduction between Pre- and Post-implementation periods from call to arrival of the ambulance at scene.
Conclusions: This is the first study to develop, implement and evaluate the impact of a training package for EMDs with
the aim of improving the recognition of stroke. Training led to a significant increase in the proportion of stroke patients dispatched as such by EMDs; a small reduction in time from call to arrival at scene by the ambulance also appeared likely. The training package has been endorsed by the UK Stroke Forum Education and Training, and is free to access on-line
Elucidation of Hepatitis C Virus Transmission and Early Diversification by Single Genome Sequencing
A precise molecular identification of transmitted hepatitis C virus (HCV) genomes could illuminate key aspects of transmission biology, immunopathogenesis and natural history. We used single genome sequencing of 2,922 half or quarter genomes from plasma viral RNA to identify transmitted/founder (T/F) viruses in 17 subjects with acute community-acquired HCV infection. Sequences from 13 of 17 acute subjects, but none of 14 chronic controls, exhibited one or more discrete low diversity viral lineages. Sequences within each lineage generally revealed a star-like phylogeny of mutations that coalesced to unambiguous T/F viral genomes. Numbers of transmitted viruses leading to productive clinical infection were estimated to range from 1 to 37 or more (median = 4). Four acutely infected subjects showed a distinctly different pattern of virus diversity that deviated from a star-like phylogeny. In these cases, empirical analysis and mathematical modeling suggested high multiplicity virus transmission from individuals who themselves were acutely infected or had experienced a virus population bottleneck due to antiviral drug therapy. These results provide new quantitative and qualitative insights into HCV transmission, revealing for the first time virus-host interactions that successful vaccines or treatment interventions will need to overcome. Our findings further suggest a novel experimental strategy for identifying full-length T/F genomes for proteome-wide analyses of HCV biology and adaptation to antiviral drug or immune pressures
Signal transducer and activator of transcription 3-mediated CD133 up-regulation contributes to promotion of hepatocellular carcinoma
published_or_final_versio
Laminar flows - Subcellular positioning of small molecules
Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/62911/1/4111016a0.pd
An Introductory Guide to Aligning Networks Using SANA, the Simulated Annealing Network Aligner.
Sequence alignment has had an enormous impact on our understanding of biology, evolution, and disease. The alignment of biological networks holds similar promise. Biological networks generally model interactions between biomolecules such as proteins, genes, metabolites, or mRNAs. There is strong evidence that the network topology-the "structure" of the network-is correlated with the functions performed, so that network topology can be used to help predict or understand function. However, unlike sequence comparison and alignment-which is an essentially solved problem-network comparison and alignment is an NP-complete problem for which heuristic algorithms must be used.Here we introduce SANA, the Simulated Annealing Network Aligner. SANA is one of many algorithms proposed for the arena of biological network alignment. In the context of global network alignment, SANA stands out for its speed, memory efficiency, ease-of-use, and flexibility in the arena of producing alignments between two or more networks. SANA produces better alignments in minutes on a laptop than most other algorithms can produce in hours or days of CPU time on large server-class machines. We walk the user through how to use SANA for several types of biomolecular networks
Robust Detection of Hierarchical Communities from Escherichia coli Gene Expression Data
Determining the functional structure of biological networks is a central goal
of systems biology. One approach is to analyze gene expression data to infer a
network of gene interactions on the basis of their correlated responses to
environmental and genetic perturbations. The inferred network can then be
analyzed to identify functional communities. However, commonly used algorithms
can yield unreliable results due to experimental noise, algorithmic
stochasticity, and the influence of arbitrarily chosen parameter values.
Furthermore, the results obtained typically provide only a simplistic view of
the network partitioned into disjoint communities and provide no information of
the relationship between communities. Here, we present methods to robustly
detect coregulated and functionally enriched gene communities and demonstrate
their application and validity for Escherichia coli gene expression data.
Applying a recently developed community detection algorithm to the network of
interactions identified with the context likelihood of relatedness (CLR)
method, we show that a hierarchy of network communities can be identified.
These communities significantly enrich for gene ontology (GO) terms, consistent
with them representing biologically meaningful groups. Further, analysis of the
most significantly enriched communities identified several candidate new
regulatory interactions. The robustness of our methods is demonstrated by
showing that a core set of functional communities is reliably found when
artificial noise, modeling experimental noise, is added to the data. We find
that noise mainly acts conservatively, increasing the relatedness required for
a network link to be reliably assigned and decreasing the size of the core
communities, rather than causing association of genes into new communities.Comment: Due to appear in PLoS Computational Biology. Supplementary Figure S1
was not uploaded but is available by contacting the author. 27 pages, 5
figures, 15 supplementary file
Differential effects of the APOE e4 allele on different domains of cognitive ability across the life-course
The association between APOE genotype and cognitive function suggests a positive role for the e2 allele and a negative role for the e4 allele. Both alleles have relatively low frequencies in the general population; hence, meta-analyses have been based on many small, heterogeneous studies. Here, we report the APOE-cognition associations in the largest single analysis to date. APOE status and cognitive ability were measured in 18 337 participants from the Generation Scotland study between 2006 and 2011. The age range was 18-94 years with a mean of 47 (SD 15). Four cognitive domains were assessed: verbal declarative memory (paragraph recall), processing speed (digit symbol substitution), verbal fluency (phonemic verbal fluency), and vocabulary (Mill Hill synonyms). Linear regression was used to assess the associations between APOE genetic status and cognition. Possession of the e4 allele was associated with lower scores on the measures of memory and processing speed in subjects aged >60. Across all age ranges, the e4 allele was linked to better verbal fluency scores. In younger subjects (≤60 years) the e4 allele was linked to higher vocabulary scores. There were no associations between the e2 allele and cognitive ability. As seen in previous meta-analyses, the APOE e4 allele is linked to poorer cognitive performance in the domains of memory and processing speed. By contrast, positive associations were seen between the e4 allele and measures of verbal fluency and vocabulary. All associations were relatively small and, in many cases, nominally significant despite the very large sample size
Individual rules for trail pattern formation in Argentine ants (Linepithema humile)
We studied the formation of trail patterns by Argentine ants exploring an
empty arena. Using a novel imaging and analysis technique we estimated
pheromone concentrations at all spatial positions in the experimental arena and
at different times. Then we derived the response function of individual ants to
pheromone concentrations by looking at correlations between concentrations and
changes in speed or direction of the ants. Ants were found to turn in response
to local pheromone concentrations, while their speed was largely unaffected by
these concentrations. Ants did not integrate pheromone concentrations over
time, with the concentration of pheromone in a 1 cm radius in front of the ant
determining the turning angle. The response to pheromone was found to follow a
Weber's Law, such that the difference between quantities of pheromone on the
two sides of the ant divided by their sum determines the magnitude of the
turning angle. This proportional response is in apparent contradiction with the
well-established non-linear choice function used in the literature to model the
results of binary bridge experiments in ant colonies (Deneubourg et al. 1990).
However, agent based simulations implementing the Weber's Law response function
led to the formation of trails and reproduced results reported in the
literature. We show analytically that a sigmoidal response, analogous to that
in the classical Deneubourg model for collective decision making, can be
derived from the individual Weber-type response to pheromone concentrations
that we have established in our experiments when directional noise around the
preferred direction of movement of the ants is assumed.Comment: final version, 9 figures, submitted to Plos Computational Biology
(accepted
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